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KMID : 1144820220280010034
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대한의생명과학회지
2022 Volume.28 No. 1 p.34 ~ p.41
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Process Optimization of Nypa fruticans Wurmb Extract using Mixed Solvent and its Functional Component Analysis
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Kim Jin
Kim Han-Sung
Son Jang-Wan
Moon Seong-Yong
Lee Sook-Young
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Abstract
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In this study, conditions for extraction of functional component from Nypa fruticans wurmb was optimized. The yield by extraction with 30% ethanol (LE30, 42.12%) was higher than those hot water extraction (LDW 33.32%), 50% ethanol (LE50, 40.12%) and 70% ethanol (LE70, 34.5%). The extract was purified and analyzed by GC MS. The prevailing compounds found in extract were Cyclodecasiloxane-, pentadecanoic acid, -eicosane, undecanal and tridecanoic acid. The presence of saturated and unsaturated fatty acids in ethanolic extract vindicate the use of this plant to treat many diseases in traditional medicine. The total phenolic contents in the LDW, LE30, LE50, LE70 extract were 128±1.65 mg/g, 205±2.3 mg/g, 210±4.23 mg/g and 180±5.6 mg/g, respectively. The DPPH was highest in LE70 extract (1,000 μg/mL, 81.14%), ABTS was highest in LE50 extract (1,000 μg/mL, 84.14%). The protective effects against oxidative stress in raw 264.7 cell imparted by the LE50 extract was better than those imparted by the other extracts. The findings of the present study suggest that 50% ethanol is best solvent for extraction of Nypa fruticans Wurmb, considering yield, polyphenol content, and antioxidant activities with extraction cost.
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KEYWORD
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Nypa fruticans Wurmb, Phytochemical, Antioxidant, Cell viability
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